Less-toxic rearrangement products of NX-toxins are formed during storage and food processing.
Identifieur interne : 000831 ( Main/Exploration ); précédent : 000830; suivant : 000832Less-toxic rearrangement products of NX-toxins are formed during storage and food processing.
Auteurs : Elisabeth Varga [Autriche] ; Gerlinde Wiesenberger [Autriche] ; Lydia Woelflingseder [Autriche] ; Krisztian Twaruschek [Autriche] ; Christian Hametner [Autriche] ; Marta Vaclaviková [Autriche] ; Alexandra Malachová [Autriche] ; Doris Marko [Autriche] ; Franz Berthiller [Autriche] ; Gerhard Adam [Autriche]Source :
- Toxicology letters [ 1879-3169 ] ; 2018.
Descripteurs français
- KwdFr :
- Cellules HT29 (MeSH), Concentration en ions d'hydrogène (MeSH), Contamination des aliments (analyse), Côlon (cytologie), Côlon (effets des médicaments et des substances chimiques), Fusarium (croissance et développement), Fusarium (métabolisme), Grains comestibles (composition chimique), Grains comestibles (microbiologie), Humains (MeSH), Manipulation des aliments (MeSH), Microbiologie alimentaire (MeSH), Relation structure-activité (MeSH), Stockage des aliments (MeSH), Structure moléculaire (MeSH), Survie cellulaire (effets des médicaments et des substances chimiques), Température élevée (MeSH), Trichothécènes (composition chimique), Trichothécènes (isolement et purification), Trichothécènes (toxicité).
- MESH :
- analyse : Contamination des aliments.
- composition chimique : Grains comestibles, Trichothécènes.
- croissance et développement : Fusarium.
- cytologie : Côlon.
- effets des médicaments et des substances chimiques : Côlon, Survie cellulaire.
- isolement et purification : Trichothécènes.
- microbiologie : Grains comestibles.
- métabolisme : Fusarium.
- toxicité : Trichothécènes.
- Cellules HT29, Concentration en ions d'hydrogène, Humains, Manipulation des aliments, Microbiologie alimentaire, Relation structure-activité, Stockage des aliments, Structure moléculaire, Température élevée.
English descriptors
- KwdEn :
- Cell Survival (drug effects), Colon (cytology), Colon (drug effects), Edible Grain (chemistry), Edible Grain (microbiology), Food Contamination (analysis), Food Handling (MeSH), Food Microbiology (MeSH), Food Storage (MeSH), Fusarium (growth & development), Fusarium (metabolism), HT29 Cells (MeSH), Hot Temperature (MeSH), Humans (MeSH), Hydrogen-Ion Concentration (MeSH), Molecular Structure (MeSH), Structure-Activity Relationship (MeSH), Trichothecenes (chemistry), Trichothecenes (isolation & purification), Trichothecenes (toxicity).
- MESH :
- chemical , chemistry : Trichothecenes.
- analysis : Food Contamination.
- chemistry : Edible Grain.
- cytology : Colon.
- drug effects : Cell Survival, Colon.
- growth & development : Fusarium.
- chemical , isolation & purification : Trichothecenes.
- metabolism : Fusarium.
- microbiology : Edible Grain.
- chemical , toxicity : Trichothecenes.
- Food Handling, Food Microbiology, Food Storage, HT29 Cells, Hot Temperature, Humans, Hydrogen-Ion Concentration, Molecular Structure, Structure-Activity Relationship.
Abstract
A new type A trichothecene mycotoxin, NX-2, was previously reported to be produced by North American isolates of the cereal pathogen Fusarium graminearum. Here we describe the isolation and structural characterization of a rearrangement product, called NX2-M1, and related compounds with different acetylation patterns (NX3-M1 and NX4-M1). In the NX-M1 derivatives, the epoxide ring is opened, and a covalent bridge between C-10 and C-12 of the trichothecene backbone is formed. In vitro translation assays showed that NX3-M1 is less toxic for eukaryotic ribosomes than NX-3. NX3-M1 also has a greatly reduced cytotoxic potential on two tested human colon cell lines. Formation of NX3-M1 can therefore be regarded as a detoxification reaction. The related F. graminearum mycotoxin deoxynivalenol (DON), which is frequently occurring worldwide, is very stable during food processing. Testing NX-3 at different pH-values and temperature conditions, as well as under conditions that simulate the storage of infected grains and bread-making process, revealed a strongly reduced stability of NX-3 and concurrent formation of NX3-M1. Although the NX-3 formed in planta is as toxic as DON, the extensive formation of the non-toxic rearrangement product should be taken into account for risk assessment of this emerging food contaminant.
DOI: 10.1016/j.toxlet.2017.12.016
PubMed: 29277571
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Less-toxic rearrangement products of NX-toxins are formed during storage and food processing.</title><author><name sortKey="Varga, Elisabeth" sort="Varga, Elisabeth" uniqKey="Varga E" first="Elisabeth" last="Varga">Elisabeth Varga</name><affiliation wicri:level="1"><nlm:affiliation>Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: elisabeth.varga@boku.ac.at.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln</wicri:regionArea><wicri:noRegion>Tulln</wicri:noRegion></affiliation></author><author><name sortKey="Wiesenberger, Gerlinde" sort="Wiesenberger, Gerlinde" uniqKey="Wiesenberger G" first="Gerlinde" last="Wiesenberger">Gerlinde Wiesenberger</name><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: gerlinde.wiesenberger@boku.ac.at.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln</wicri:regionArea><wicri:noRegion>Tulln</wicri:noRegion></affiliation></author><author><name sortKey="Woelflingseder, Lydia" sort="Woelflingseder, Lydia" uniqKey="Woelflingseder L" first="Lydia" last="Woelflingseder">Lydia Woelflingseder</name><affiliation wicri:level="3"><nlm:affiliation>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria; Department of Food Chemistry and Toxicology, University of Vienna, Vienna, Austria. Electronic address: lydia.woelflingseder@univie.ac.at.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria; Department of Food Chemistry and Toxicology, University of Vienna, Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Twaruschek, Krisztian" sort="Twaruschek, Krisztian" uniqKey="Twaruschek K" first="Krisztian" last="Twaruschek">Krisztian Twaruschek</name><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: krisztian.twaruschek@boku.ac.at.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln</wicri:regionArea><wicri:noRegion>Tulln</wicri:noRegion></affiliation></author><author><name sortKey="Hametner, Christian" sort="Hametner, Christian" uniqKey="Hametner C" first="Christian" last="Hametner">Christian Hametner</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Applied Synthetic Chemistry, Vienna University of Technology, Vienna, Austria. Electronic address: christian.hametner@tuwien.ac.at.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Institute of Applied Synthetic Chemistry, Vienna University of Technology, Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Vaclavikova, Marta" sort="Vaclavikova, Marta" uniqKey="Vaclavikova M" first="Marta" last="Vaclaviková">Marta Vaclaviková</name><affiliation wicri:level="1"><nlm:affiliation>Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: marta.vaclavikova@boku.ac.at.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln</wicri:regionArea><wicri:noRegion>Tulln</wicri:noRegion></affiliation></author><author><name sortKey="Malachova, Alexandra" sort="Malachova, Alexandra" uniqKey="Malachova A" first="Alexandra" last="Malachová">Alexandra Malachová</name><affiliation wicri:level="1"><nlm:affiliation>Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: alexandra.malachova@boku.ac.at.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln</wicri:regionArea><wicri:noRegion>Tulln</wicri:noRegion></affiliation></author><author><name sortKey="Marko, Doris" sort="Marko, Doris" uniqKey="Marko D" first="Doris" last="Marko">Doris Marko</name><affiliation wicri:level="3"><nlm:affiliation>Department of Food Chemistry and Toxicology, University of Vienna, Vienna, Austria. Electronic address: doris.marko@univie.ac.at.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Food Chemistry and Toxicology, University of Vienna, Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Berthiller, Franz" sort="Berthiller, Franz" uniqKey="Berthiller F" first="Franz" last="Berthiller">Franz Berthiller</name><affiliation wicri:level="1"><nlm:affiliation>Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: franz.berthiller@boku.ac.at.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln</wicri:regionArea><wicri:noRegion>Tulln</wicri:noRegion></affiliation></author><author><name sortKey="Adam, Gerhard" sort="Adam, Gerhard" uniqKey="Adam G" first="Gerhard" last="Adam">Gerhard Adam</name><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: gerhard.adam@boku.ac.at.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln</wicri:regionArea><wicri:noRegion>Tulln</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29277571</idno><idno type="pmid">29277571</idno><idno type="doi">10.1016/j.toxlet.2017.12.016</idno><idno type="wicri:Area/Main/Corpus">000969</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000969</idno><idno type="wicri:Area/Main/Curation">000969</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000969</idno><idno type="wicri:Area/Main/Exploration">000969</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Less-toxic rearrangement products of NX-toxins are formed during storage and food processing.</title><author><name sortKey="Varga, Elisabeth" sort="Varga, Elisabeth" uniqKey="Varga E" first="Elisabeth" last="Varga">Elisabeth Varga</name><affiliation wicri:level="1"><nlm:affiliation>Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: elisabeth.varga@boku.ac.at.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln</wicri:regionArea><wicri:noRegion>Tulln</wicri:noRegion></affiliation></author><author><name sortKey="Wiesenberger, Gerlinde" sort="Wiesenberger, Gerlinde" uniqKey="Wiesenberger G" first="Gerlinde" last="Wiesenberger">Gerlinde Wiesenberger</name><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: gerlinde.wiesenberger@boku.ac.at.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln</wicri:regionArea><wicri:noRegion>Tulln</wicri:noRegion></affiliation></author><author><name sortKey="Woelflingseder, Lydia" sort="Woelflingseder, Lydia" uniqKey="Woelflingseder L" first="Lydia" last="Woelflingseder">Lydia Woelflingseder</name><affiliation wicri:level="3"><nlm:affiliation>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria; Department of Food Chemistry and Toxicology, University of Vienna, Vienna, Austria. Electronic address: lydia.woelflingseder@univie.ac.at.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria; Department of Food Chemistry and Toxicology, University of Vienna, Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Twaruschek, Krisztian" sort="Twaruschek, Krisztian" uniqKey="Twaruschek K" first="Krisztian" last="Twaruschek">Krisztian Twaruschek</name><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: krisztian.twaruschek@boku.ac.at.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln</wicri:regionArea><wicri:noRegion>Tulln</wicri:noRegion></affiliation></author><author><name sortKey="Hametner, Christian" sort="Hametner, Christian" uniqKey="Hametner C" first="Christian" last="Hametner">Christian Hametner</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Applied Synthetic Chemistry, Vienna University of Technology, Vienna, Austria. Electronic address: christian.hametner@tuwien.ac.at.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Institute of Applied Synthetic Chemistry, Vienna University of Technology, Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Vaclavikova, Marta" sort="Vaclavikova, Marta" uniqKey="Vaclavikova M" first="Marta" last="Vaclaviková">Marta Vaclaviková</name><affiliation wicri:level="1"><nlm:affiliation>Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: marta.vaclavikova@boku.ac.at.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln</wicri:regionArea><wicri:noRegion>Tulln</wicri:noRegion></affiliation></author><author><name sortKey="Malachova, Alexandra" sort="Malachova, Alexandra" uniqKey="Malachova A" first="Alexandra" last="Malachová">Alexandra Malachová</name><affiliation wicri:level="1"><nlm:affiliation>Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: alexandra.malachova@boku.ac.at.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln</wicri:regionArea><wicri:noRegion>Tulln</wicri:noRegion></affiliation></author><author><name sortKey="Marko, Doris" sort="Marko, Doris" uniqKey="Marko D" first="Doris" last="Marko">Doris Marko</name><affiliation wicri:level="3"><nlm:affiliation>Department of Food Chemistry and Toxicology, University of Vienna, Vienna, Austria. Electronic address: doris.marko@univie.ac.at.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Food Chemistry and Toxicology, University of Vienna, Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Berthiller, Franz" sort="Berthiller, Franz" uniqKey="Berthiller F" first="Franz" last="Berthiller">Franz Berthiller</name><affiliation wicri:level="1"><nlm:affiliation>Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: franz.berthiller@boku.ac.at.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln</wicri:regionArea><wicri:noRegion>Tulln</wicri:noRegion></affiliation></author><author><name sortKey="Adam, Gerhard" sort="Adam, Gerhard" uniqKey="Adam G" first="Gerhard" last="Adam">Gerhard Adam</name><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: gerhard.adam@boku.ac.at.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln</wicri:regionArea><wicri:noRegion>Tulln</wicri:noRegion></affiliation></author></analytic><series><title level="j">Toxicology letters</title><idno type="eISSN">1879-3169</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Survival (drug effects)</term><term>Colon (cytology)</term><term>Colon (drug effects)</term><term>Edible Grain (chemistry)</term><term>Edible Grain (microbiology)</term><term>Food Contamination (analysis)</term><term>Food Handling (MeSH)</term><term>Food Microbiology (MeSH)</term><term>Food Storage (MeSH)</term><term>Fusarium (growth & development)</term><term>Fusarium (metabolism)</term><term>HT29 Cells (MeSH)</term><term>Hot Temperature (MeSH)</term><term>Humans (MeSH)</term><term>Hydrogen-Ion Concentration (MeSH)</term><term>Molecular Structure (MeSH)</term><term>Structure-Activity Relationship (MeSH)</term><term>Trichothecenes (chemistry)</term><term>Trichothecenes (isolation & purification)</term><term>Trichothecenes (toxicity)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cellules HT29 (MeSH)</term><term>Concentration en ions d'hydrogène (MeSH)</term><term>Contamination des aliments (analyse)</term><term>Côlon (cytologie)</term><term>Côlon (effets des médicaments et des substances chimiques)</term><term>Fusarium (croissance et développement)</term><term>Fusarium (métabolisme)</term><term>Grains comestibles (composition chimique)</term><term>Grains comestibles (microbiologie)</term><term>Humains (MeSH)</term><term>Manipulation des aliments (MeSH)</term><term>Microbiologie alimentaire (MeSH)</term><term>Relation structure-activité (MeSH)</term><term>Stockage des aliments (MeSH)</term><term>Structure moléculaire (MeSH)</term><term>Survie cellulaire (effets des médicaments et des substances chimiques)</term><term>Température élevée (MeSH)</term><term>Trichothécènes (composition chimique)</term><term>Trichothécènes (isolement et purification)</term><term>Trichothécènes (toxicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Trichothecenes</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Contamination des aliments</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Food Contamination</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Edible Grain</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Grains comestibles</term><term>Trichothécènes</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Fusarium</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Côlon</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Colon</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Survival</term><term>Colon</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Côlon</term><term>Survie cellulaire</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Fusarium</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Trichothecenes</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Trichothécènes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fusarium</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Grains comestibles</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Edible Grain</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Fusarium</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Trichothecenes</term></keywords><keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Trichothécènes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Food Handling</term><term>Food Microbiology</term><term>Food Storage</term><term>HT29 Cells</term><term>Hot Temperature</term><term>Humans</term><term>Hydrogen-Ion Concentration</term><term>Molecular Structure</term><term>Structure-Activity Relationship</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules HT29</term><term>Concentration en ions d'hydrogène</term><term>Humains</term><term>Manipulation des aliments</term><term>Microbiologie alimentaire</term><term>Relation structure-activité</term><term>Stockage des aliments</term><term>Structure moléculaire</term><term>Température élevée</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A new type A trichothecene mycotoxin, NX-2, was previously reported to be produced by North American isolates of the cereal pathogen Fusarium graminearum. Here we describe the isolation and structural characterization of a rearrangement product, called NX2-M1, and related compounds with different acetylation patterns (NX3-M1 and NX4-M1). In the NX-M1 derivatives, the epoxide ring is opened, and a covalent bridge between C-10 and C-12 of the trichothecene backbone is formed. In vitro translation assays showed that NX3-M1 is less toxic for eukaryotic ribosomes than NX-3. NX3-M1 also has a greatly reduced cytotoxic potential on two tested human colon cell lines. Formation of NX3-M1 can therefore be regarded as a detoxification reaction. The related F. graminearum mycotoxin deoxynivalenol (DON), which is frequently occurring worldwide, is very stable during food processing. Testing NX-3 at different pH-values and temperature conditions, as well as under conditions that simulate the storage of infected grains and bread-making process, revealed a strongly reduced stability of NX-3 and concurrent formation of NX3-M1. Although the NX-3 formed in planta is as toxic as DON, the extensive formation of the non-toxic rearrangement product should be taken into account for risk assessment of this emerging food contaminant.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29277571</PMID><DateCompleted><Year>2018</Year><Month>02</Month><Day>21</Day></DateCompleted><DateRevised><Year>2018</Year><Month>02</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-3169</ISSN><JournalIssue CitedMedium="Internet"><Volume>284</Volume><PubDate><Year>2018</Year><Month>Mar</Month><Day>01</Day></PubDate></JournalIssue><Title>Toxicology letters</Title><ISOAbbreviation>Toxicol Lett</ISOAbbreviation></Journal><ArticleTitle>Less-toxic rearrangement products of NX-toxins are formed during storage and food processing.</ArticleTitle><Pagination><MedlinePgn>205-212</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0378-4274(17)31526-6</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.toxlet.2017.12.016</ELocationID><Abstract><AbstractText>A new type A trichothecene mycotoxin, NX-2, was previously reported to be produced by North American isolates of the cereal pathogen Fusarium graminearum. Here we describe the isolation and structural characterization of a rearrangement product, called NX2-M1, and related compounds with different acetylation patterns (NX3-M1 and NX4-M1). In the NX-M1 derivatives, the epoxide ring is opened, and a covalent bridge between C-10 and C-12 of the trichothecene backbone is formed. In vitro translation assays showed that NX3-M1 is less toxic for eukaryotic ribosomes than NX-3. NX3-M1 also has a greatly reduced cytotoxic potential on two tested human colon cell lines. Formation of NX3-M1 can therefore be regarded as a detoxification reaction. The related F. graminearum mycotoxin deoxynivalenol (DON), which is frequently occurring worldwide, is very stable during food processing. Testing NX-3 at different pH-values and temperature conditions, as well as under conditions that simulate the storage of infected grains and bread-making process, revealed a strongly reduced stability of NX-3 and concurrent formation of NX3-M1. Although the NX-3 formed in planta is as toxic as DON, the extensive formation of the non-toxic rearrangement product should be taken into account for risk assessment of this emerging food contaminant.</AbstractText><CopyrightInformation>Copyright © 2017 The Author(s). Published by Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Varga</LastName><ForeName>Elisabeth</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: elisabeth.varga@boku.ac.at.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wiesenberger</LastName><ForeName>Gerlinde</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: gerlinde.wiesenberger@boku.ac.at.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Woelflingseder</LastName><ForeName>Lydia</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria; Department of Food Chemistry and Toxicology, University of Vienna, Vienna, Austria. Electronic address: lydia.woelflingseder@univie.ac.at.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Twaruschek</LastName><ForeName>Krisztian</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: krisztian.twaruschek@boku.ac.at.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hametner</LastName><ForeName>Christian</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Institute of Applied Synthetic Chemistry, Vienna University of Technology, Vienna, Austria. Electronic address: christian.hametner@tuwien.ac.at.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vaclaviková</LastName><ForeName>Marta</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: marta.vaclavikova@boku.ac.at.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Malachová</LastName><ForeName>Alexandra</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: alexandra.malachova@boku.ac.at.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Marko</LastName><ForeName>Doris</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Food Chemistry and Toxicology, University of Vienna, Vienna, Austria. Electronic address: doris.marko@univie.ac.at.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Berthiller</LastName><ForeName>Franz</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: franz.berthiller@boku.ac.at.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Adam</LastName><ForeName>Gerhard</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. Electronic address: gerhard.adam@boku.ac.at.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>12</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Toxicol Lett</MedlineTA><NlmUniqueID>7709027</NlmUniqueID><ISSNLinking>0378-4274</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014255">Trichothecenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C000608633">trichothecene NX-2</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002470" MajorTopicYN="N">Cell Survival</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003106" MajorTopicYN="N">Colon</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002523" MajorTopicYN="Y">Edible Grain</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005506" MajorTopicYN="N">Food Contamination</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005511" MajorTopicYN="Y">Food Handling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005516" MajorTopicYN="N">Food Microbiology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D061353" MajorTopicYN="N">Food Storage</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005670" MajorTopicYN="N">Fusarium</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019073" MajorTopicYN="N">HT29 Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006358" MajorTopicYN="N">Hot Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006863" MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015394" MajorTopicYN="N">Molecular Structure</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013329" MajorTopicYN="N">Structure-Activity Relationship</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014255" MajorTopicYN="Y">Trichothecenes</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Alamar Blue assay</Keyword><Keyword MajorTopicYN="N">Detoxification</Keyword><Keyword MajorTopicYN="N">Fusarium graminearum</Keyword><Keyword MajorTopicYN="N">Sulforhodamine B assay</Keyword><Keyword MajorTopicYN="N">Trichothecene</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>11</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2017</Year><Month>12</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>12</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>12</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>2</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>12</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29277571</ArticleId><ArticleId IdType="pii">S0378-4274(17)31526-6</ArticleId><ArticleId IdType="doi">10.1016/j.toxlet.2017.12.016</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Autriche</li></country><region><li>Vienne (Autriche)</li></region><settlement><li>Vienne (Autriche)</li></settlement></list><tree><country name="Autriche"><noRegion><name sortKey="Varga, Elisabeth" sort="Varga, Elisabeth" uniqKey="Varga E" first="Elisabeth" last="Varga">Elisabeth Varga</name></noRegion><name sortKey="Adam, Gerhard" sort="Adam, Gerhard" uniqKey="Adam G" first="Gerhard" last="Adam">Gerhard Adam</name><name sortKey="Berthiller, Franz" sort="Berthiller, Franz" uniqKey="Berthiller F" first="Franz" last="Berthiller">Franz Berthiller</name><name sortKey="Hametner, Christian" sort="Hametner, Christian" uniqKey="Hametner C" first="Christian" last="Hametner">Christian Hametner</name><name sortKey="Malachova, Alexandra" sort="Malachova, Alexandra" uniqKey="Malachova A" first="Alexandra" last="Malachová">Alexandra Malachová</name><name sortKey="Marko, Doris" sort="Marko, Doris" uniqKey="Marko D" first="Doris" last="Marko">Doris Marko</name><name sortKey="Twaruschek, Krisztian" sort="Twaruschek, Krisztian" uniqKey="Twaruschek K" first="Krisztian" last="Twaruschek">Krisztian Twaruschek</name><name sortKey="Vaclavikova, Marta" sort="Vaclavikova, Marta" uniqKey="Vaclavikova M" first="Marta" last="Vaclaviková">Marta Vaclaviková</name><name sortKey="Wiesenberger, Gerlinde" sort="Wiesenberger, Gerlinde" uniqKey="Wiesenberger G" first="Gerlinde" last="Wiesenberger">Gerlinde Wiesenberger</name><name sortKey="Woelflingseder, Lydia" sort="Woelflingseder, Lydia" uniqKey="Woelflingseder L" first="Lydia" last="Woelflingseder">Lydia Woelflingseder</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/DetoxFungiV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000831 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000831 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= DetoxFungiV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:29277571
|texte= Less-toxic rearrangement products of NX-toxins are formed during storage and food processing.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:29277571" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a DetoxFungiV1
| This area was generated with Dilib version V0.6.38. |  |